谁了解sacpic 染色方法的全过程，我想知道Sacpic 染色的具体步骤和各个步骤的原理，各种染色剂染色的部位

发布网友发布时间：2022-04-30 07:54

共1个回答

热心网友时间：2023-10-21 11:21

http://translate.googleusercontent.com/translate_c?hl=zh-CN&langpair=en|zh-CN&u=http://www.scribd.com/doc/35251876/319/SACPIC-staining-method&rurl=translate.google.com&usg=ALkJrhhJ-rroo_LbT7mrwq7f_nnTZWjnaQ
有步骤，看第266页，要等一会，但是没有原理
我看不太懂，不管有用没用你看看吧
8.3.3 SACPIC staining method 8.3.3.1 Celestin blue 10g Iron alum (Ammonium ferric sulfate dodecahydrate, NH 4 Fe(SO 4 ) 2 . 12H 2 O) (BDH chemicals Ltd, England)
Dissolved in 200mL RO water
Mix overnight
Add 1.0g Celestin blue powder (Fluka, Switzerland)
Heat slowly in flask (watching for froth)
Boil for 3 min
Cool
Filter into 250mL container
Add 6.8mL Glycerol (#100-GLEP.2500, Pure science, New Zealand)
Shake well
R.L. Edmonds 266 8.3.3.2Scott’s tap water 2 g/L Sodium bicarbonate 20 g/L Magnesium Sulfate 8.3.3.3 Safranine solution Add the following ingredients in order and mix for one hour
2g Safranine (BDH Chemical Ltd, England)
49mL Absolute ethanol
49mL RO water
8.3.3.4Picric acid in ethanol 0.5 ML Picric acid (Saturated aqueous solution) 50mL Absolute alcohol mix 8.3.3.5 Picro-indigo-carmine 0.125g Indigo Carmine powder (Sigma, USA)
50mLPicric acid (Saturated aqueous solution)
mix
8.3.3.6SACPIC staining protocol Water rinse Celestin blue solution 5 min
Water rinse
Haematoxylin
5 min (Mayers Hamalaun solution, Applichem, Germany)
Water rinse
Scott’s “tap water”
2 min
Water rinse
Safranine solution
5 min
70% Ethanol
Absolute ethanol
Picric acid in ethanol
2 min 95% Ethanol

热心网友时间：2023-10-21 11:21