发布网友 发布时间:2022-07-29 18:55
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热心网友 时间:2023-11-28 10:01
Abstract : Objective : To establish Huaimi Rutin Determination by HPLC. Methods : Shimadzu ODS column (5 μ m, 150 mm × 4.6 mm) as the stationary phase, methanol-1% acetic acid (40:60) as the mobile phase was 35. C, the detection wavelength 257nm; flow rate of 1.0ml/min. Results : Huaimi rutin and other components separated from the baseline, 0.024-0.056mg/ml in the context of influent concentration area with peaks value was linear relations Department of regression equation : Y=3E+07X-94270, 0.9990, The average recovery of 98.44%. Conclusion : The method is simple with high accuracy and high separation efficiency, suitable for Huaimi Rutin Determination. Keywords : Huaimi; Rutin; HPLC; Determination