spike-in control翻译

发布网友发布时间：2022-10-01 22:02

共1个回答

热心网友时间：2024-11-26 17:02

http://www.dxy.cn/bbs/topic/20565318
An
RNA
spike-in
is
an
RNA
transcript
used
to
calibrate
measurements
in
a
DNA
microarray
experiment.
Each
spike-in
is
designed
to
hybridize
with
a
specific
control
probe
on
the
target
array.
Manufacturers
of
commercially
available
microarrays
typically
offer
companion
RNA
spike-ins
"kits".[1][2][3][4]
Known
amounts
of
RNA
spike-ins
are
mixed
with
the
experiment
sample
ring
preparation.
Subsequently
the
measured
degree
of
hybridization
between
the
spike-ins
and
the
control
probes
is
used
to
normalize
the
hybridization
measurements
of
the
sample
RNA.
RNA
spike-in是用于对DNA微阵列试验进行校正的DNA转录物，这种spike-in可以和标靶阵列的特异性控制（对照）探针进行杂交。商业化微阵列扫描仪的商家一般会同时提供的RNA
spike-in试剂盒。
制备样品时，将已知量的RNA
spike-in和试验样本混合，然后检测spike-in和控制（对照）探针的杂交程度，用这样的数据对来样品RNA的杂交水平数值进行校正。