谁有做酵母双杂交用的PGADT7的空载质粒啊?跪求

发布网友发布时间：2022-04-23 06:45

共1个回答

热心网友时间：2022-06-16 22:19

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基本信息
别名:pGADT7-AD
启动子:ADH1
复制子:2μ ori,ori
质粒分类:酵母系列，酵母双杂交载体
质粒大小:7987bp
原核抗性:氨苄青霉素Amp
筛选标记:LEU2
克隆菌株:大肠杆菌DH5α
培养条件:37℃，有氧 LB
表达宿主:酵母细胞
5'测序引物:T7:TAATACGACTCACTATAGGG
3'测序引物:根据序列设计引物
质粒简介
pGADT7 AD is a yeast expression vector that is designed to express a protein of interestfused to a GAL4 activation domain (AD; amino acids 768–881). Transcription of the GAL4 ADfusion is driven by the constitutively active ADH1 promoter (PADH1), and is terminated at theADH1 transcription termination signal (TADH1). The GAL4 AD fusion contains an N-terminalSV40 nuclear localization signal (SV40 NLS; 1) that targets the protein to the yeast nucleus,and a hemagglutinin epitope tag (HA Tag), located between the GAL4 AD and the proteinof interest, that allows the protein to be easily detected with HA-tag antibodies.
质粒图谱