翻译一下摘要啊急救

发布网友发布时间：2022-07-13 10:13

共1个回答

热心网友时间：2023-10-27 21:49

AFP is a kind of isolated from the hemolymph of polar fish to the protein. AFP can not according to the number of the lower the freezing point of water, is capable of binding to inhibit ice crystal growth in the ice surface to avoid cell by physical damage. The sequence analysis of AFP. With the five kinds of fish antifreeze protein multiple sequence comparison, constructing the phylogenetic tree. The recombinant plasmid containing AFP-PPIC9K of Escherichia coli bacteria after activation and shake, plasmid DNA was extracted by alkaline lysis method. The restriction endonuclease SacI its linearization using agarose gel electrophoresis, the recombinant plasmid was linearized with the degree of agarose gel electrophoresis, the Recombinant Plasmid Extraction Kit linearization. Prepared yeast KM71 state, by electroporation has been linearized plasmids into yeast KM71 state of feeling. Coating on the lack of histidine in YNB medium. The experimental results show that: the protein belongs to the third type antifreeze protein, having a molecular weight of 9911Da; a theoretical isoelectric point of 7.72; unstable coefficient is 28.46, which belongs to the stable protein, the protein is localized in the extracellular, close evolutionary relationship with americanus. Taking PPIC9K as the carrier of the antifreeze protein AFP into the yeast KM71, obtained more strain for expression and purification of foundation inced next in selective culture medium after.