做细胞爬片的免疫荧光染色，bsa封闭后，敷一抗时怎么处理啊？2

发布网友发布时间：2023-10-14 08:53

共1个回答

热心网友时间：2024-12-12 12:43

1. Dilute your primary antibody in your blocking buffer
2. Incubate your coverslips with primary antibody for 1 hours at room temperature or 4 degrees overnight
3. wash 3x PBS
4.your sample is ready for secondary antibody

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